Draft Genome Sequences of Anaplasma marginale Strains MEX-15-099-01 and MEX-31-096-01, Two Mexican Isolates with Different Degrees of Virulence.

Fernando Martínez-Ocampo,Mayra Cobaxin Cárdenas,Edgar Dantán-González,Itzel Amaro-Estrada,Rosa Estela Quiroz-Castañeda,Sergio Rodríguez-Camarillo,Christina A Cuomo

doi:10.1128/mra.01184-19

Abstract

Anaplasma marginale is an intraerythrocytic bacterium that causes bovine anaplasmosis and is endemic in Mexico. In this work, we report two draft genome sequences of Mexican isolates from different geographical regions and with different degrees of virulence.

Highlights

Anaplasma marginale is an intraerythrocytic rickettsial Gram-negative bacterium that causes clinical signs of bovine anaplasmosis, namely, fever, anemia, jaundice, weakness, and even respiratory distress
Contigs of two Mexican strains were differentiated from contigs that belong to other organisms based on GϩC content of each contig assessed using a Python script
We aligned the sequences of each contig assembled with the NCBI Nucleotide database using the BLASTN suite (8) and “Anaplasma marginale” as the organism name

Summary

Introduction

Anaplasma marginale is an intraerythrocytic rickettsial Gram-negative bacterium that causes clinical signs of bovine anaplasmosis, namely, fever, anemia, jaundice, weakness, and even respiratory distress. The Illumina adapter sequences were removed from paired-end reads using the ILLUMINACLIP trimming step of Trimmomatic (version 0.36), with default settings (5). Low-quality bases were removed using the dynamictrim algorithm of the SolexaQAϩϩ suite (version 3.1.7.1) (6) with a Phred quality score (Q) of Ͻ13. The resulting paired-end reads were de novo assembled using SPAdes (version 3.11.1) (7) with the following options: (i) only run the assembly module (-only-assembler), (ii) reduce the number of mismatches (-careful), and (iii) k-mer lengths between 21 and 127.

Results

Conclusion