Abstract

ABSTRACTSpore-forming microorganisms are of concern for forward contamination because they can survive harsh interplanetary travel. Here, we report the draft genome sequences of 12 spore-forming strains isolated from the Manned Spacecraft Operations Building (MSOB) and the Vehicle Assembly Building (VAB) in Cape Canaveral, FL, where the Viking spacecraft were assembled.

Highlights

  • Spore-forming microorganisms are of concern for forward contamination because they can survive harsh interplanetary travel

  • Strain V1-29 had genes coding for cobalamin synthase and lipoteichoic acid primase (LtaP), which are essential in the lipoteichoic acid synthesis pathway [3]

  • Strain ATCC 29669 had a uniquely present gene coding for glycogen branching enzyme GH-57 archaeal type, which was previously isolated in a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1T [13]

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Summary

Introduction

Spore-forming microorganisms are of concern for forward contamination because they can survive harsh interplanetary travel. Strain V3-13 had putative genes coding for clustered regularly interspaced short palindromic repeat (CRISPR)-associated proteins Csn1 and Cas2. Strain V1-29 had genes coding for cobalamin synthase and lipoteichoic acid primase (LtaP), which are essential in the lipoteichoic acid synthesis pathway [3].

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