Abstract
Here, we report the draft genome sequences obtained for 10 bacterial strains isolated from root nodules of Alnus trees. These members of the nodule microbiome were sequenced to determine their potential functional roles in plant health. The selected strains belong to the genera Rhodococcus, Kocuria, Rothia, Herbaspirillum, Streptomyces, and Thiopseudomonas.
Highlights
Root nodule samples were collected from Alnus trees found by Adam’s Pond at Jackson’s Laboratory in Durham, NH
The root nodules were surface sterilized with hydrogen peroxide and rinsed several times with sterile distilled water
10 isolates were chosen for whole-genome sequencing analysis to provide insight into their plant-microbe interactions, including potential plant growth-promoting activity
Summary
Actinorhizal plants form a symbiotic fixing actinobacterial genus Frankia association that allows with these members plants to of the nitrogencolonize stressed environments [1–6]. The last wash of the sterilized nodule was incubated in LB medium to ensure that all epiphytes associated with the plant were removed. The nodule was cut into a fine powder with a sterilized razor, and dilutions were plated onto Czapeck and R2A media containing cycloheximide and nalidixic acid. About 60 isolates were initially obtained, purified, and propagated on either Czapeck or R2A medium (Table 1).
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