Abstract
In this study, we report the draft genome sequence of Zygosaccharomyces mellis CA-7, isolated from purchased honey imported from Canada. The 10.19-Mb genome contains 4,963 gene models. To our knowledge, this annotated genome sequence is the first from the species Z. mellis and will contribute to a better understanding of the osmotolerance of microorganisms in high-sugar products.
Highlights
In this study, we report the draft genome sequence of Zygosaccharomyces mellis CA-7, isolated from purchased honey imported from Canada
Z. mellis was cultured in yeast malt (YM) medium containing 50% glucose or 1% glucose at 28°C for 12 hours on a shaker at 160 rpm
The paired-end reads were subjected to quality trimming and assembled using CLC Genomics Workbench (Qiagen) version 11.0.1 with default parameter settings
Summary
We report the draft genome sequence of Zygosaccharomyces mellis CA-7, isolated from purchased honey imported from Canada. To obtain the draft genome sequence of Z. mellis CA-7, the DNA was extracted using the benzyl chloride method (4). We performed transcriptome sequencing (RNA-seq) analysis to obtain information on gene expression in hyperosmotic medium. Z. mellis was cultured in YM medium containing 50% glucose (pH 5.0) or 1% glucose (pH 5.0) at 28°C for 12 hours on a shaker at 160 rpm.
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