Abstract
ABSTRACTVibrio penaeicida is a bacterial pathogen of cultured shrimp. The draft genome sequence of V. penaeicida strain TUMSAT-NU1 consists of 100 scaffolds with a total of 6.41 Mbp. We identified possible virulence factors, and we found that V. penaeicida and Vibrio nigripulchritudo are closely related.
Highlights
Vibrio penaeicida is a bacterial pathogen of cultured shrimp
To search for bacterial genomes that are closely related to V. penaeicida TUMSAT-NU1, we used NCBI BLAST with several V. penaeicida TUMSAT-NU1 scaffolds as queries against the NCBI nonredundant nucleotide database
A preliminary search with NCBI BLAST revealed that V. penaeicida TUMSAT-NU1 is closely related (80% to 90% nucleotide identity) to V. nigripulchritudo, another shrimpinfecting Vibrio [12]
Summary
Vibrio penaeicida is a bacterial pathogen of cultured shrimp. The draft genome sequence of V. penaeicida strain TUMSAT-NU1 consists of 100 scaffolds with a total of 6.41 Mbp. We present the draft genome sequence of V. penaeicida strain TUMSAT-NU1 [5], which was originally isolated from a diseased shrimp in the 1980s in Japan. We extracted the bacterial genomic DNA using the standard cetyltrimethylammonium bromide (CTAB) method and constructed a paired-end DNA library with a Nextera XT library preparation kit (Illumina, USA). We sequenced the library with the MiSeq platform and MiSeq reagent kit version 2 (300 cycles).
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