Abstract

ABSTRACTWe report the draft genome sequence of Vibrio chagasii strain 18LP, isolated from gilthead seabream larvae at a fish hatchery research station in Portugal. The genome presents numerous features underlying opportunistic behavior, including genes coding for toxin biosynthesis and tolerance, host cell invasion, and heavy metal resistance.

Highlights

  • Vibrio spp. are ubiquitous in the marine environment and may cause disease outbreaks, leading to high economic losses in aquaculture [1, 2]

  • The read quality was enhanced by trimming the low-quality bases using the “Trim sequences” option in CLC Genomics Workbench v7.0.4

  • The sequence reads were assembled into 49 contigs with the “de novo assembly” option within CLC Genomics Workbench v7.0.4

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Summary

Introduction

Vibrio spp. are ubiquitous in the marine environment and may cause disease outbreaks, leading to high economic losses in aquaculture [1, 2]. A genome library was constructed using the Illumina Nextera XT DNA library preparation kit (insert length, 450 bp). Paired-end sequencing was conducted on the Illumina HiSeq 2500 platform at BaseClear (The Netherlands). FASTQ sequence files were generated using the Illumina Casava v1.8.3 pipeline.

Results
Conclusion
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