Abstract
Enterococcus faecium is a leading cause of health care-associated infections, with specific lineages circulating in hospital settings worldwide. Here, we report the draft genome sequence of the multidrug-resistant and biofilm-producing E. faecium UEL170, sequence type 412 (ST412), isolated from an inpatient with a urinary tract infection. This strain is a member of clonal complex 17 (CC17), a globally hospital-associated clone.
Highlights
Enterococcus faecium is a leading cause of health care-associated infections, with specific lineages circulating in hospital settings worldwide
For DNA isolation, 5 CFU was inoculated into brain heart infusion (BHI) broth (HiMedia, Brazil), and the culture was incubated for 24 h at 37°C
The genome was annotated with Rapid Annotation using System Technology (RAST) v2.0 using FIGfams technology release 70 according to the default software recommendations [16]
Summary
Enterococcus faecium is a leading cause of health care-associated infections, with specific lineages circulating in hospital settings worldwide. For DNA isolation, 5 CFU was inoculated into brain heart infusion (BHI) broth (HiMedia, Brazil), and the culture was incubated for 24 h at 37°C. Harvested by centrifugation (2,600 ϫ g for 10 min at 10°C) and washed twice with 0.15 phosphate-saline buffer at pH 7.2.
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