Abstract
Nostoc sp. strain KVJ20 was isolated from the symbiotic organs of the liverwort Blasia pusilla This cyanobacterium has been shown to have broad symbiotic competence, and bacterial extracts have inhibitory effects on cancer cell lines and microbes. An array of genes for the production of secondary metabolites is present.
Highlights
Nostoc sp. strain KVJ20 was isolated from the symbiotic organs of the liverwort Blasia pusilla
KVJ20 was purified according to the method for bacterial genomic DNA isolation using CTAB, v3 [5]
The library was sequenced on a MiSeq machine (Illumina) at UiT The Artic University of Norway using the MiSeq reagent kit v3 (2 ϫ 300 bp; catalog number MS-102-3003)
Summary
Nostoc sp. strain KVJ20 was isolated from the symbiotic organs of the liverwort Blasia pusilla. KVJ20 was purified according to the method for bacterial genomic DNA isolation using CTAB, v3 [5]. The only modification is two washes in 5 M NaCl preceding the DNA isolation.
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