Abstract
The draft genome sequence of the type strain Saccharopolyspora hirsuta subsp. hirsuta VKM Ac-666 was sequenced. This moderately thermophilic actinobacterial strain of sugarcane bagasse origin is able to transform different steroid substrates.
Highlights
Actinobacteria from different habitats were described to utilize steroids as ubiquitous growth substrates or to perform structural modifications of natural or synthetic steroids as a prelude to their further full degradation as a carbon and energy source
The strain S. hirsuta subsp. hirsuta VKM Ac-666T was obtained from the All-Russian Collection of Microorganisms (VKM) of the G.K
The remaining reads were trimmed by quality scores using BBDuk v38.35 (10)
Summary
Actinobacteria from different habitats were described to utilize steroids as ubiquitous growth substrates or to perform structural modifications of natural or synthetic steroids as a prelude to their further full degradation as a carbon and energy source. Genomic DNA extraction from S. hirsuta subsp. Hirsuta VKM Ac-666T was carried out as described (8) with the following modifications.
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