Abstract
Streptococcus salivarius is a prevalent commensal species of human oral mucosal surfaces. S. salivarius strain HS0302 produces the type AII lantibiotic salivaricin A2. Here, we report its draft genome sequence, revealing its potential to produce a variety of bacteriocins.
Highlights
Streptococcus salivarius is a prevalent commensal species of human oral mucosal surfaces
Streptococcus salivarius HS0302 was cultured on a Todd-Hewitt yeast extract (THyex) agar plate or THyex broth
A single colony of the strain was inoculated into 10 ml THyex broth and was incubated overnight
Summary
Streptococcus salivarius is a prevalent commensal species of human oral mucosal surfaces. Streptococcus salivarius HS0302 was cultured on a Todd-Hewitt yeast extract (THyex) agar plate (containing 30 g/liter Todd-Hewitt broth, 3 g/liter yeast extract, and 15 g/liter agar) or THyex broth. Genomic DNA was isolated from the overnight culture using the Wizard genomic DNA purification kit (Promega) following the manufacturer’s instructions. Whole-genome sequencing was performed using the Illumina paired-end 150 platform (Novogene, Beijing, China).
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