Abstract
Herpomyces periplanetae is an obligate biotroph of Periplaneta americana, the American cockroach. Its nearly cosmopolitan distribution is shaped by its globally invasive host and the international pet trade. Here, we report the draft genome sequence of H. periplanetae, based on a thallus from P. americana collected in Cambridge, Massachusetts.
Highlights
Herpomyces periplanetae is an obligate biotroph of Periplaneta americana, the American cockroach
Identity and DNA isolation were validated by the amplification and sequencing of the following three ribosomal DNA fragments [3, 12]: the small subunit (SSU) (GenBank accession number MG438331), internal transcribed spacer (ITS) (MG438309), and large subunit (LSU) (MG438359)
Low-quality sequences were not trimmed, because this resulted in a poorer assembly
Summary
Herpomyces periplanetae is an obligate biotroph of Periplaneta americana, the American cockroach. Processing (A260/A280 ϭ 1.79, A260/A230 ϭ 2.01), and the gDNA concentration as measured by the Qubit was 4.62 ng/l. Identity and DNA isolation were validated by the amplification and sequencing of the following three ribosomal DNA fragments [3, 12]: the small subunit (SSU) (GenBank accession number MG438331), internal transcribed spacer (ITS) (MG438309), and large subunit (LSU) (MG438359). Library preparation was done using the PrepX DNA library kit on the Apollo NGS library prep system (TaKaRa Bio, Mountain View, CA) following the manufacturer’s specifications and with 50 l of gDNA as the starting volume.
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