Abstract
ABSTRACTThe spread of multidrug-resistant (MDR) tuberculosis (TB) has become a major global challenge. In 2016, Tasmania recorded its first known incidence of MDR-TB. Here, we report the draft whole-genome sequence of the Mycobacterium tuberculosis isolate from this case, TASMDR1, and describe single-nucleotide polymorphisms associated with its drug resistance.
Highlights
The earliest written record of tuberculosis (TB) in Tasmania comes from Colonel David Collins, who reported in 1804 that a member of his Hobart settlement had consumption [1]
Assembled contigs were ordered with respect to the M. tuberculosis H37Rv genome using ABACAS [11]
A total of 1,553 variant sites were identified relative to the H37Rv genome and consisted of 1,408 single-nucleotide variants (SNVs) and 145 insertions/deletions
Summary
The earliest written record of tuberculosis (TB) in Tasmania comes from Colonel David Collins, who reported in 1804 that a member of his Hobart settlement had consumption [1]. We have previously characterized the genomes of MDR and extensively drug-resistant (XDR) Mycobacterium tuberculosis [3,4,5]. Genomic DNA of the Tasmanian isolate, TASMDR1, was sequenced using an Illumina MiSeq instrument. A total of 2,860,297 paired-end reads were mapped to the publicly available annotated genome of M. tuberculosis reference strain H37Rv (GenBank accession number NC_000962.3) [6] by Burrows-Wheeler alignment [7].
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