Draft Genome Sequence of the Endophytic Bacterium Variovorax paradoxus KB5, Which Has Antagonistic Activity against a Phytopathogen, Pseudomonas syringae pv. tomato DC3000.

Chi Eun Hong,Jeong Mee Park,Sung Hee Jo,Haeyoung Jeong,Ick-Hyun Jo

doi:10.1128/genomea.00950-17

Abstract

ABSTRACTVariovorax paradoxus KB5, isolated from the inside of Arabidopsis thaliana leaves, showed antibacterial activity against the phytopathogen Pseudomonas syringae pv. tomato DC3000. Here, we report a draft genome sequence of V. paradoxus KB5, which contains a delftibactin-like nonribosomal peptide biosynthetic gene cluster.

Highlights

Variovorax paradoxus KB5, isolated from the inside of Arabidopsis thaliana leaves, showed antibacterial activity against the phytopathogen Pseudomonas syringae pv. tomato DC3000
CP002417 [6]) to be the closest neighbor of KB5, the average nucleotide identity for 40 phylogenetic marker genes, calculated using specI (7), was only 93.4%, which was below the cutoff value for allocation into a species cluster
Rapid Annotations using Subsystems Technology (RAST) predicted the presence of a mechanism that assembles siderophores (5)

Summary

Introduction

Variovorax paradoxus KB5, isolated from the inside of Arabidopsis thaliana leaves, showed antibacterial activity against the phytopathogen Pseudomonas syringae pv. tomato DC3000. The Variovorax paradoxus KB5 genome was sequenced using the Illumina HiSeq 2500 platform at the National Instrument Center for Environmental Management at Seoul National University (Seoul, Republic of Korea). 1,465,897,430 paired reads (151 cycles) were generated in a library (average insert size of ϳ458 bp) constructed using the TruSeq Nano LT DNA sample preparation version 2.

Results

Conclusion