Abstract
Clavibacter sp. strain CF11, which was isolated from soil at a tomato-planting greenhouse in Inner Mongolia, North China, has a high capability for producing cold-active cellulase at low temperatures. Here, we report the draft genome sequence of strain CF11, which comprises 2,437 protein-coding sequences and 49 RNA-coding sequences.
Highlights
Cellulose is one of the most promising alternatives to fossil fuels and is one of the most abundant and renewable biopolymers on the earth
Cellulose degradation under low temperature accounts for a large proportion of the carbon cycle in nature (1), while cold-active cellulase may contribute to energy saving and is very attractive in some application fields at low temperatures (2)
The strain shows a high carboxymethylcellulose (CMC) enzyme activity (56 mol·ml-1·min-1 at 10°C; optimal, 60 mol·ml-1·min-1 at 22°C), which is different from the optimal activity of about 37°C seen in other Clavibacter spp. (3), and it has potential application at low temperatures
Summary
Cellulose is one of the most promising alternatives to fossil fuels and is one of the most abundant and renewable biopolymers on the earth. Cellulose degradation under low temperature accounts for a large proportion of the carbon cycle in nature (1), while cold-active cellulase may contribute to energy saving and is very attractive in some application fields at low temperatures (2). We isolated a cellulolytic and low-temperature-tolerant bacterium, strain CF11, belonging to Clavibacter, on 16S rRNA and gyrB genotyping, from soil at a tomato-planting greenhouse in Inner Mongolia, North China. The 16S rRNA gene sequence of CF11 shows 98.3% identity to that of the Clavibacter michiganensis strain LMG 3663.
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