Abstract

Clostridium clariflavum strain 4-2a, a novel strain isolated from a thermophilic biocompost pile, has demonstrated an extensive capability to utilize both cellulose and hemicellulose under thermophilic anaerobic conditions. Here, we report the draft genome of this strain.

Highlights

  • The ability of thermophilic clostridia to extensively degrade lignocellulosic materials and produce fermentation products that may serve as biocommodities makes them excellent candidates for consolidating bioprocessing applications [1]

  • The genome sequence of Clostridium clariflavum DSM 19732 revealed novel mechanisms among thermophilic clostridia to break down cellulose and hemicellulose [2]

  • The draft genome was generated at the DOE Joint Genome Institute (JGI) using Illumina data [5] from a short-insert paired-end library with an average insert size of 270 bp, generating 24,015,970 reads, and an Illumina long-insert paired-end library with an average insert size of 6,484.62 bp, generating 25,583,980 reads and totaling 7,440 Mbp of Illumina data

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Summary

Introduction

The ability of thermophilic clostridia to extensively degrade lignocellulosic materials and produce fermentation products that may serve as biocommodities makes them excellent candidates for consolidating bioprocessing applications [1]. The genome sequence of Clostridium clariflavum DSM 19732 revealed novel mechanisms among thermophilic clostridia to break down cellulose and hemicellulose [2]. C. clariflavum strain 4-2a is a novel strain isolated from compost [3], which has demonstrated the additional ability to utilize xylose and considerably degrade unpretreated switchgrass [4].

Results
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