Abstract
Stenotrophomonas maltophilia is a nosocomial pathogen that primarily causes respiratory infection in humans. This pathogen is widely distributed in the environment, including in foods. Here, we report the draft genome sequence of S. maltophilia strain CRB139-1, isolated from poultry meat in Japan. The genome size was 4,619,918 bp at 90× coverage.
Highlights
Stenotrophomonas maltophilia is a nosocomial pathogen that primarily causes respiratory infection in humans
The reads were trimmed using Qiagen CLC Genomics Workbench v.11.0 and assembled de novo to the contig and scaffold levels using CGE Assembler v.1.2 and CONTIGuator v.2 [7, 8] with the reference genome sequence of strain NCTC13014, as this was shown to be the closest relative of CRB139-1 (Fig. 1)
S. maltophilia is known to be intrinsically resistant to carbapenems via -lactamase production or multidrug efflux pumps [10]
Summary
Stenotrophomonas maltophilia is a nosocomial pathogen that primarily causes respiratory infection in humans. Genomic DNA was extracted from a bacterial culture grown in lysogeny broth agar (Becton, Dickinson) using the Maxwell RSC blood DNA kit (Promega), and the library was prepared using the Ion Xpress Plus fragment library kit (Life Technologies). Genome sequencing was performed using the Ion Torrent Chef/GeneStudio S5 system (Life Technologies). The reads were trimmed using Qiagen CLC Genomics Workbench v.11.0 and assembled de novo to the contig and scaffold levels using CGE Assembler v.1.2 and CONTIGuator v.2 [7, 8] with the reference genome sequence of strain NCTC13014, as this was shown to be the closest relative of CRB139-1 (Fig. 1).
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