Draft Genome Sequence of Shewanella sp. Strain P1-14-1, a Bacterial Inducer of Settlement and Morphogenesis in Larvae of the Marine Hydroid Hydractinia echinata.

Maja Rischer,Huijuan Guo,Jon Clardy,Jonathan L Klassen,Ekaterina Shelest,Thomas Wolf,Christine Beemelmanns

doi:10.1128/genomea.00003-16

Maja Rischer, Huijuan Guo + Show 5 more

Open Access

https://doi.org/10.1128/genomea.00003-16

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Abstract

The assembly and annotation of the draft genome sequence of Shewanella sp. strain P1-14-1 are reported here to investigate the genes responsible for interkingdom interactions, secondary metabolite production, and microbial electrogenesis.

Highlights

Members of the genus Shewanella are facultative anaerobic bacteria colonizing mostly marine and freshwater environments [1, 2] and have been shown to be metal-reducing bacteria capable of transferring electrons to the environment by different mechanisms [3]
In our quest to isolate and characterize chemical cues involved in the larval settlement of Hydractinia echinata [6] and to identify the responsible genes and regulation mechanisms, we isolated, assessed, and sequenced marine microbes commonly associated with H. echinata [7, 8]
Shewanella sp. strain P1-14-1 was isolated from the tissue from a feeding polyp of the marine hydroid H. echinata, purchased from the Marine Biological Laboratory in Woods Hole, MA

Summary

Introduction

Members of the genus Shewanella are facultative anaerobic bacteria colonizing mostly marine and freshwater environments [1, 2] and have been shown to be metal-reducing bacteria capable of transferring electrons to the environment by different mechanisms [3]. Besides their bioremediation capabilities and electrogenic potential, Shewanella spp. are known to produce secondary metabolites and induce settlement in larvae of biofouling marine invertebrates [4, 5]. In our quest to isolate and characterize chemical cues involved in the larval settlement of Hydractinia echinata [6] and to identify the responsible genes and regulation mechanisms, we isolated, assessed, and sequenced marine microbes commonly associated with H. echinata [7, 8]. We first tested larval settlement and morphogenic activity using a larva-based assay, and its antimicrobial activity was assessed.

Results

Conclusion