Abstract
Members of the phylum Planctomycetes, which are capable of surviving in a wide range of environments, are some of the least-explored bacteria. Here, we report the near-complete draft genome sequence and annotation of the planctomycete Rhodopirellula baltica BR-MGV, which was isolated from the soil of a contaminated Brazilian mangrove.
Highlights
Members of the phylum Planctomycetes, which are capable of surviving in a wide range of environments, are some of the least-explored bacteria
The enriched beads were sequenced on the Ion Torrent (PGM) platform using 316 chips and an Ion PGM 400 sequencing kit
Genome annotation was performed with PATRIC version 3.3.15, which identified 6,644 coding sequences (CDS) and 49 predicted noncoding RNAs (45 tRNAs and 4 rRNAs)
Summary
Members of the phylum Planctomycetes, which are capable of surviving in a wide range of environments, are some of the least-explored bacteria. The genome sequencing of R. baltica strain BR-MGV was performed using the Ion Torrent PGM platform (Thermo Fisher Scientific). The extracted genomic DNA was enzymatically fragmented and ligated to the appropriate adapters using the Ion Xpress Plus fragment library kit following the manufacturer’s protocol. The obtained fragments were ligated to the ionic beads and amplified by emulsion PCR using the Ion PGM template OT2 400 kit.
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