Abstract
We report the draft genome of Raoultella ornithinolytica TNT, a Gram-negative bacterium of the Enterobacteriaceae isolated from military soil in Belgium. Strain TNT uses nitrite released from trinitrotoluene (TNT) for growth and is a potent plant growth promoter. An analysis of its 5.6-Mb draft genome will bring insights into TNT degradation-reinforcing bioremediation applications.
Highlights
We report the draft genome of Raoultella ornithinolytica TNT, a Gram-negative bacterium of the Enterobacteriaceae isolated from military soil in Belgium
A TNT-detoxifying strain was isolated from TNT-contaminated forest soil at a military site in Belgium
Identified as Raoultella ornithinolytica B6 by partial 16S rRNA gene sequencing and phenotypic profiling, the closest related partial 16S rRNA gene sequence (97.8%) was from strain B6
Summary
We report the draft genome of Raoultella ornithinolytica TNT, a Gram-negative bacterium of the Enterobacteriaceae isolated from military soil in Belgium. Trinitrotoluene (TNT)-contaminated sites pose risks to human and environmental health, making remediation a priority. Detoxification by indigenous bacteria or use of TNT-transforming bacteria in bioaugmented rhizoremediation is preferred to invasive and costly excavation or incineration techniques. A TNT-detoxifying strain was isolated from TNT-contaminated forest soil at a military site in Belgium.
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