Abstract
Here, we describe the draft genome sequence of Raoultella ornithinolytica P079F W, isolated from the feces of an infant residing in a neonatal intensive care unit during an ongoing study to characterize the neonate gut microbiota. P079F W will be used in studies investigating the role of the microbiome in neonatal infections.
Highlights
We describe the draft genome sequence of Raoultella ornithinolytica P079F W, isolated from the feces of an infant residing in a neonatal intensive care unit during an ongoing study to characterize the neonate gut microbiota
DNA was extracted from an overnight culture of P079F W using a phenol-chloroform method described fully by Kiu et al [6] and sequenced using the 96-plex Illumina HiSeq 2500 platform to generate 1,092,878 125-bp paired-end reads [7]
Raw data provided by the sequencing center were checked using FastQC v0.11.4; no adapter trimming was required, and reads had an average Phred score of Ͼ25
Summary
We describe the draft genome sequence of Raoultella ornithinolytica P079F W, isolated from the feces of an infant residing in a neonatal intensive care unit during an ongoing study to characterize the neonate gut microbiota. After storage at Ϫ80°C, the fecal sample was diluted 1:10 in buffer and plated onto MacConkey agar no. Strain P079F W was isolated along with strain P079F P [5] from the fecal sample. Both isolates were identified by phenotypic testing as Klebsiella oxytoca [API20E code 524577(3/7)].
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