Abstract
Here, we report a draft genome sequence of Ralstonia sp. strain SET104, isolated from the root nodules of Aeschynomene indica. The assembled draft genome size was 4,796,748 bp, containing a predicted total of 4,464 protein-encoding sequences. SET104 appears to be a novel species of the genus Ralstonia.
Highlights
Aeschynomene indica is known to form nitrogen-fixing root and stem nodules in association with bacteria in the family Bradyrhizobiaceae [1, 2] through intercellular infection [1, 3] that is independent of nodulation (Nod) factors [4, 5]
Genomic DNA was extracted from strain SET104 cells using a genomic DNA purification kit (Promega, USA) following the manufacturer’s protocol
The resulting draft genome of strain SET104 consisted of 4,796,748 bases with a GC content of 63.3%
Summary
Aeschynomene indica is known to form nitrogen-fixing root and stem nodules in association with bacteria in the family Bradyrhizobiaceae [1, 2] through intercellular infection [1, 3] that is independent of nodulation (Nod) factors [4, 5]. Genomic DNA was extracted from strain SET104 cells using a genomic DNA purification kit (Promega, USA) following the manufacturer’s protocol. DNA libraries were prepared using a TruSeq DNA sample prep kit (Illumina, USA) and sequenced with the NextSeq 500 sequencer (Illumina, USA).
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