Draft Genome Sequence of Probiotic Lactobacillus brevis TUCO-5E, Isolated from Porcine Milk.

Sandra Rayen Quilodran-Vega,Elvira Maria Hebert,Haruki Kitazawa,Julio Villena,Alexis Salas-Burgos,Leonardo Albarracin,Alexis Fonseca,Lucila Saavedra

doi:10.1128/mra.01239-18

Sandra Rayen Quilodran-Vega, Elvira Maria Hebert + Show 6 more

Open Access

https://doi.org/10.1128/mra.01239-18

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Abstract

This report describes the draft genome sequence of Lactobacillus brevis TUCO-5E, a probiotic strain isolated from porcine maternal milk. The reads were generated by a whole-genome sequencing (WGS) strategy on an Illumina MiSeq sequencer and were assembled into contigs with a total estimated size of 2,461,089 bp. A total of 2,455 open reading frames (ORFs) were predicted, including 2,301 protein-coding sequences. The draft genome sequence of L. brevis TUCO-5E will be useful for further studies of specific genetic features and for understanding the mechanisms of its probiotic properties in the porcine host.

Highlights

This report describes the draft genome sequence of Lactobacillus brevis TUCO-5E, a probiotic strain isolated from porcine maternal milk
L. brevis TUCO-5E was cultured for 12 h at 37°C in de Man-RogosaSharpe (MRS) broth (Oxoid, Cambridge, UK), and genomic DNA isolation was performed as described in reference 2
The Rapid Annotations using Subsystems Technology (RAST) server was used for functional annotation of predicted genes (4)

Summary

Introduction

This report describes the draft genome sequence of Lactobacillus brevis TUCO-5E, a probiotic strain isolated from porcine maternal milk. A single colony of the TUCO-5E strain was picked for culturing prior to DNA isolation. L. brevis TUCO-5E was cultured for 12 h at 37°C (final log phase) in de Man-RogosaSharpe (MRS) broth (Oxoid, Cambridge, UK), and genomic DNA isolation was performed as described in reference 2.

Results

Conclusion