Draft genome sequence of Phomopsis longicolla isolate MSPL 10-6.

Shuxian Li,Leslie L Domier,Burton H Bluhm,Pingsheng Ji,Nadim Alkharouf,Ning Zhang,Benjamin Matthews,Omar Darwish

doi:10.1016/j.gdata.2014.11.007

Abstract

Phomopsis longicolla is the primary cause of Phomopsis seed decay in soybean. This disease severely affects soybean seed quality by reducing seed viability and oil content, altering seed composition, and increasing frequencies of moldy and/or split beans. It is one of the most economically important soybean diseases. Here, we report the de novo assembled draft genome sequence of the P. longicolla isolate MSPL10-6, which was isolated from field-grown soybean seed in Mississippi, USA. This study represents the first reported genome sequence of a seedborne fungal pathogen in the Diaporthe–Phomopsis complex. The P. longicolla genome sequence will enable research into the genetic basis of fungal infection of soybean seed and provide information for the study of soybean–fungal interactions. The genome sequence will also be valuable for molecular genetic marker development, manipulation of pathogenicity-related genes and development of new control strategies for this pathogen.

Highlights

Phomopsis longicolla is the primary cause of Phomopsis seed decay in soybean
We report the de novo assembled draft genome sequence of the P. longicolla isolate MSPL10-6, which was isolated from field-grown soybean seed in Mississippi, USA
Phomopsis longicolla MSPL 10-6 Illumina HiSeq 2500 sequencer Raw and processed DNA extracted from a wild-type strain, no treatment Genome sequencing n/a Soybean field in Stoneville, Mississippi, USA

Summary

Data in Brief

Shuxian Li a,⁎, Omar Darwish b, Nadim Alkharouf b, Benjamin Matthews c, Pingsheng Ji d, Leslie L. Phomopsis longicolla is the primary cause of Phomopsis seed decay in soybean. This disease severely affects soybean seed quality by reducing seed viability and oil content, altering seed composition, and increasing frequencies of moldy and/or split beans. We report the de novo assembled draft genome sequence of the P. longicolla isolate MSPL10-6, which was isolated from field-grown soybean seed in Mississippi, USA. The P. longicolla genome sequence will enable research into the genetic basis of fungal infection of soybean seed and provide information for the study of soybean–fungal interactions. Phomopsis longicolla MSPL 10-6 Illumina HiSeq 2500 sequencer Raw and processed DNA extracted from a wild-type strain, no treatment Genome sequencing n/a Soybean field in Stoneville, Mississippi, USA

Materials and methods

Processed data

Findings

Data analysis and results