Draft Genome Sequence of Phenylobacterium immobile Strain E (DSM 1986), Isolated from Uncontaminated Soil in Ecuador.

Ondrej Reznicek,Francisca Luesken,Bernhard Hauer,Sandra J Facey

doi:10.1128/genomea.00420-15

Ondrej Reznicek, Francisca Luesken + Show 2 more

Open Access

https://doi.org/10.1128/genomea.00420-15

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Abstract

We report the draft genome sequence of 3.3 Mb and the sequence (19.2 kb) of a natural plasmid isolated from Phenylobacterium immobile strain E (DSM 1986), able to degrade xenobiotic compounds as the sole carbon source. The sequences reveal a large number of novel Rieske nonheme iron aromatic ring-hydroxylating oxygenases (RHOs).

Highlights

We report the draft genome sequence of 3.3 Mb and the sequence (19.2 kb) of a natural plasmid isolated from Phenylobacterium immobile strain E (DSM 1986), able to degrade xenobiotic compounds as the sole carbon source
Herbicides such as chloridazon or aromatic compounds of medical use, such as the xenobiotics antipyrine and diclofenac, are considered as pollutants when exposed to the environment (1)
Phenylobacterium immobile (DSM 1986), a soil bacterium whose strains are found on various continents (3) and is capable of degrading the xenobiotic compounds chloridazon and antipyrine as the sole carbon source (4), has been examined for its enzymatic content to explore novel ring-hydroxylating oxygenases (RHOs)

Summary

Introduction

We report the draft genome sequence of 3.3 Mb and the sequence (19.2 kb) of a natural plasmid isolated from Phenylobacterium immobile strain E (DSM 1986), able to degrade xenobiotic compounds as the sole carbon source. Microbial degradation of such pollutants often occurs via initial hydroxylation of the phenolic moiety using dioxygenases (DOs) to make it enzymatically accessible for further degradation (2). Phenylobacterium immobile (DSM 1986), a soil bacterium whose strains are found on various continents (3) and is capable of degrading the xenobiotic compounds chloridazon and antipyrine as the sole carbon source (4), has been examined for its enzymatic content to explore novel ring-hydroxylating oxygenases (RHOs). The draft genome of P. immobile strain E was obtained using Illumina shotgun and mate-pair sequencing, which resulted in paired-end reads of 250 bp.

Results

Conclusion