Abstract
Nitrosomonas sp. strain APG5 (=NCIMB 14870 = ATCC TSA-116) was isolated from dry beach sand collected from a supralittoral zone of the northwest coast of the United States. The draft genome sequence revealed that it represents a new species of the cluster 6 Nitrosomonas spp. that is closely related to Nitrosomonas ureae and Nitrosomonas oligotropha.
Highlights
Nitrosomonas sp. strain APG5 was isolated from dry beach sand collected from a supralittoral zone of the northwest coast of the United States
The draft genome was initially annotated with the Rapid Annotations using Subsystems Technology (RAST) server (11) and SEED Viewer (12) to confirm some housekeeping genes and key functional genes
Strain AL212 (GenBank accession number CP002552) (16), and Nitrosomonas sp. strain Is79 (GenBank accession number CP002876) (17) were 82.2%, 81.9%, and 77.9%, respectively, which were lower than the average nucleotide identity (ANI) value of 95%, which corresponds to a 70% species level cutoff determined by DNA-DNA hybridization (18)
Summary
Nitrosomonas sp. strain APG5 (ϭNCIMB 14870 ϭ ATCC TSA-116) was isolated from dry beach sand collected from a supralittoral zone of the northwest coast of the United States. A draft genome sequence was obtained using the Illumina MiSeq platform and Nextera XT sample preparation kit v2 with 2 ϫ 250-bp paired-end reads (3,338,462 reads). The draft genome was initially annotated with the Rapid Annotations using Subsystems Technology (RAST) server (version 2.0) (11) and SEED Viewer (12) to confirm some housekeeping genes and key functional genes.
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