Draft Genome Sequence of Multidrug-Resistant Enterococcus faecium Strain E1298, with a Sequence Type 1274 Profile, Recovered from the Cloacal Microbiome of a Tropical Screech Owl (Megascops choliba) in Rio de Janeiro, Brazil.

Andréa Andrade Rangel Freitas,Paul J Planet,Adriana Rocha Faria,Stephanie Silva Rodrigues Souza,Vânia Lúcia Carreira Merquior,Lúcia Martins Teixeira,Julie C Dunning Hotopp

doi:10.1128/mra.00168-19

Abstract

Here, we present the draft genome sequence of Enterococcus faecium strain E1298, a representative of the clonal complex 17 (CC17), identified as sequence type 1274 (ST1274) and resistant to multiple classes of antimicrobials, isolated from the cloaca of a tropical screech owl (Megascops choliba) in Rio de Janeiro, Brazil.

Highlights

We present the draft genome sequence of Enterococcus faecium strain E1298, a representative of the clonal complex 17 (CC17), identified as sequence type 1274 (ST1274) and resistant to multiple classes of antimicrobials, isolated from the cloaca of a tropical screech owl (Megascops choliba) in Rio de Janeiro, Brazil
High-quality reads were assembled by the autoassembly strategy and annotated using the RAST tool kit (RASTtk) in the genome annotation service of the Pathosystems Resource Integration Center (PATRIC 3.4.11) [11], leading to the predic
A comparison of amino acid polymorphisms detected in the C-terminal region of PBP5 with a reference sequence (GenBank accession number X84860) showed the polymorphisms Met485Ala, Ala499Thr, Glu629Val, and Pro667Ser and the insertion of serine after position 466, associated, when combined, with high-level resistance to ␤-lactam antibiotics in E. faecium strains from animals and humans [1, 12]

Summary

Introduction

We present the draft genome sequence of Enterococcus faecium strain E1298, a representative of the clonal complex 17 (CC17), identified as sequence type 1274 (ST1274) and resistant to multiple classes of antimicrobials, isolated from the cloaca of a tropical screech owl (Megascops choliba) in Rio de Janeiro, Brazil. Genomic DNA was obtained from 1.5 ml of that culture using a Wizard genomic DNA purification kit (Promega, Madison, WI, USA), prepped using the Nextera XT kit, and sequenced on a HiSeq 2500 sequencer (Illumina, Inc., San Diego, CA, USA) with 125-bp paired-end reads. A total of 344,111 paired-end Illumina reads and 51,960,761 bp were obtained.

Results

Conclusion