Abstract
We report the draft genome sequence of Microbacterium oryzae strain MB-10T, which was isolated from rice field soil. The genome is 3.04 Mb, with a G+C content of 71.10%, and encodes 2,727 proteins. The genome sequence provides further information about strain MB-10T and the genus Microbacterium.
Highlights
We report the draft genome sequence of Microbacterium oryzae strain MB-10T, which was isolated from rice field soil
De novo genome assembly of PacBio reads was performed with the Canu 1.3 assembler (7)
Comparative genomic analysis was performed with the genome sequences of type strains available in the NCBI database, to evaluate the genomic relatedness, using JSpeciesWS (10)
Summary
We report the draft genome sequence of Microbacterium oryzae strain MB-10T, which was isolated from rice field soil. Quality control of the sequencing reads was performed using the parameters correct and trim in Canu 1.3. De novo genome assembly of PacBio reads was performed with the Canu 1.3 assembler (https://github.com/marbl/canu) (parameters: correct; p, bacteria; merylMemory, 15; batThreads, 12; stopOnLowCoverage, 100; genomeSize, 3.0m) (7). The scaffolding was performed using the Single Molecular Integrative Scaffolding (SMIS) pipeline (https://github.com/fg6/smis) (parameters: score, 50; len, 2000; step, 200; contig, 3000; edge, 5).
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