Abstract
ABSTRACTThe draft genome sequence of Methylocaldum sp. 14B, an obligate methanotrophic strain isolated from solid-state anaerobic digestion systems, is reported here. Strain 14B possesses genes for methane oxidation and exhibited tolerance to H2S.
Highlights
Biogas is a renewable energy source, but its direct utilization has several limitations due to corrosion caused by H2S present in biogas and difficulty with its storage and transportation
Minisatellite DNA and microsatellite DNA were predicted based on the number and length of the repeat units
The draft genome contained four rRNA genes, 45 tRNA genes, one small RNA (sRNA) gene, 98 minisatellite DNA, 12 microsatellite DNA, 152 tandem repeat sequences, 4,586 genes with predicted functions, and 919 genes coding for hypothetical proteins
Summary
Biogas is a renewable energy source, but its direct utilization has several limitations due to corrosion caused by H2S present in biogas and difficulty with its storage and transportation. 14B was cultured in a mineral medium [3] with methane as the sole substrate. One paired-end library was prepared from genomic DNA using the NEBNext Ultra DNA library prep kit for Illumina (NEB) and sequenced (2 ϫ 300 bp) using MiSeq. Sequence reads were de novo assembled using Newbler [4], resulting in 85 contigs (N50, 133,347 bp; largest contig, 294,260 bp) with an average coverage of 90ϫ.
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