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Abstract
Methylibium sp. strain T29 was isolated from a gasoline-contaminated aquifer and proved to have excellent capabilities in degrading some common fuel oxygenates like methyl tert-butyl ether, tert-amyl methyl ether and tert-butyl alcohol along with other organic compounds. Here, we report the draft genome sequence of M. sp. strain T29 together with the description of the genome properties and its annotation. The draft genome consists of 608 contigs with a total size of 4,449,424 bp and an average coverage of 150×. The genome exhibits an average G + C content of 68.7 %, and contains 4754 protein coding and 52 RNA genes, including 48 tRNA genes. 71 % of the protein coding genes could be assigned to COG (Clusters of Orthologous Groups) categories. A formerly unknown circular plasmid designated as pT29A was isolated and sequenced separately and found to be 86,856 bp long.
Highlights
Fuel oxygenates like Methyl tert-butyl ether (MTBE), Ethyl tert-butyl ether (ETBE) and Tert-amyl methyl ether (TAME) have been blended into gasoline for decades to boost octane ratings and to improve the efficiency of fuel combustion in engines
Fuel oxygenates like MTBE, ETBE and TAME have been blended into gasoline for decades to boost octane ratings and to improve the efficiency of fuel combustion in engines
Afterwards, a number of bacteria closely related to M. petroleiphilum PM1 were detected based on 16S rDNA sequences at MTBE-contaminated sites at different geographic locations suggesting that the genus might have an important role in MTBE biodegradation [8, 9]
Summary
Fuel oxygenates like MTBE, ETBE and TAME have been blended into gasoline for decades to boost octane ratings and to improve the efficiency of fuel combustion in engines. Later its complete genome sequence was published which revealed that besides the 4 Mb circular chromosome, M. petroleiphilum PM1 possesses a ~600 kb megaplasmid carrying the genes involved in MTBE degradation [10]. As part of a FrenchHungarian project aiming to characterize novel fuel oxygenate-degrading bacteria at the genomic level, we have isolated a novel Methylibium strain.
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