Abstract
Pseudomonas fluorescens DSM 11579 is known to be a producer of the lipopeptides brabantamide and thanamycin. Its draft genome gives insight into the complete secondary metabolite production capacity of the strain and builds the basis for a comparative study with Pseudomonas sp. strain SH-C52, a lipopeptide-producing strain involved in natural disease-suppressive soils.
Highlights
As part of our ongoing efforts to investigate plant growth-promoting rhizobacteria (PGPR), one of the authors (J.M.R.) isolated the plant-associated strain Pseudomonas sp
The remaining genomic DNA (gDNA) was sheared to 10 kb and used to generate SMRTbell libraries using the standard library protocols of the Pacific Biosciences DNA template preparation kit
The continuous long-read (CLR) data were filtered by read length (Ͼ35 bp), subread length (Ͼ35 bp), and read quality (Ͼ0.75)
Summary
As part of our ongoing efforts to investigate plant growth-promoting rhizobacteria (PGPR), one of the authors (J.M.R.) isolated the plant-associated strain Pseudomonas sp. The data collected were processed and filtered using the SMRT Analysis software suite. The continuous long-read (CLR) data were filtered by read length (Ͼ35 bp), subread length (Ͼ35 bp), and read quality (Ͼ0.75). The final quality statistics included 344,142 reads with an average read length of 3,534 bp and a maximum read length of 38,722 bp.
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