Abstract
Lactobacillus paraplantarum OSY-TC318 was isolated from Turkish Tulum cheese and found to produce a potent anti-Gram-positive peptide. Sequencing of the OSY-TC318 genome revealed a genome size of 3,587,488 bp and an average GC content of 43.4%. Mining of the OSY-TC318 draft genome sequence revealed the gene cluster responsible for the biosynthesis of paraplantaricin TC318.
Highlights
When used to manufacture fermented foods, lactic acid bacteria (LAB) contribute to a product’s flavor and texture and to its safety (1, 2)
Torrent Suite 4.4.2 was used to generate raw sequence reads after trimming low-quality reads
Contig reordering was done against the reference genome of L. para
Summary
When used to manufacture fermented foods, lactic acid bacteria (LAB) contribute to a product’s flavor and texture and to its safety (1, 2). Sequencing results were analyzed using various software programs at their default settings, unless otherwise specified. Torrent Suite 4.4.2 was used to generate raw sequence reads after trimming low-quality reads. The raw data, 1,499,819 short sequencing reads (quality score, Ն15), were de novo assembled using CLC Genomics Workbench 9.0 (Qiagen) with the parameters mapping mode; minimum contig length, 200; automatic word size, 21; and automatic bubble size, 161.
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