Abstract
Lactobacillus fermentum Lf2, an Argentine cheese isolate, can produce high concentrations of exopolysaccharides (EPS). These EPS were shown to improve the texture and rheology of yogurt, as well as to play a protective role in mice exposed to Salmonella enterica serovar Typhimurium. Three gene clusters potentially involved in EPS production were identified in different locations of the L. fermentum Lf2 genome.
Highlights
Lactobacillus fermentum Lf2, an Argentine cheese isolate, can produce high concentrations of exopolysaccharides (EPS)
The genome of L. fermentum Lf2 was sequenced by GenProbio (University of Parma, Italy), using 2 ϫ 250-bp paired-end Illumina MiSeq reads
The function of each potential EPS-related gene was confirmed by considering the best hit obtained by a BLASTx search against the current NCBI database
Summary
Lactobacillus fermentum Lf2, an Argentine cheese isolate, can produce high concentrations of exopolysaccharides (EPS). The genome of L. fermentum Lf2 was sequenced by GenProbio (University of Parma, Italy), using 2 ϫ 250-bp paired-end Illumina MiSeq reads. The resulting assembly was composed of 251 contigs, with an N50 of 32,695, average nucleotide coverage of 57ϫ, GC content of 51.7%, and total length of 2.05 Mb. The draft genome was reordered using the Mauve contig mover to match the synteny of the complete genome, L. fermentum CECT5716.
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