Abstract

We report here the 4.57-Mb draft genome sequence of hydrocarbon-degrading Enterobacter cloacae strain S1:CND1 isolated from oil-contaminated soil in Guwahati, India. S1:CND1 contains 4,205 coding sequences and has a G+C content of 57.45%. This is the first report of the genome sequence of an E. cloacae adapted to an oil-contaminated environment.

Highlights

  • The genomic DNA for strain S1:CND1 was extracted using an Ultra-Clean Microbial DNA Isolation Kit (MoBio Laboratories, Carlsbad, CA, USA) according to the manufacturer’s protocol

  • E. cloacae strain S1:CND1 was isolated from oil-contaminated soil collected from the Noonmati oil refinery in Guwahati, Assam, India

  • Genome annotation for strain S1:CND1 was carried out with the NCBI Prokaryotic Genome Annotation Pipeline, which predicted the presence of 4,205 coding sequences (CDSs), along with 16 rRNAs, 77 tRNAs, 6 noncoding RNAs, and 50 pseudogenes

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Summary

Introduction

The genomic DNA for strain S1:CND1 was extracted using an Ultra-Clean Microbial DNA Isolation Kit (MoBio Laboratories, Carlsbad, CA, USA) according to the manufacturer’s protocol. There have been a few reports of hydrocarbondegrading E. cloacae strains (2), no genome sequence for any hydrocarbon-degrading E. cloacae strain is currently available. E. cloacae strain S1:CND1 was isolated from oil-contaminated soil collected from the Noonmati oil refinery in Guwahati, Assam, India.

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