Abstract
ABSTRACTHalolamina pelagica strain CDK2, a halophilic archaeon (growth range 1.36 to 5.12 M NaCl), was isolated from rhizosphere of wild grasses of hypersaline soil of the Rann of Kutch, Gujarat, India. Its draft genome contains 2,972,542 bp and 3,485 coding sequences, depicting genes for halophilic serine proteases and trehalose synthesis.
Highlights
Halolamina pelagica strain CDK2, a halophilic archaeon, was isolated from rhizosphere of wild grasses of hypersaline soil of the Rann of Kutch, Gujarat, India
Shotgun sequencing generated 11,512,861 pairedend reads (2 ϫ 250 bp), while PacBio SMRT resulted in 120,716 long reads (30 to 40 kb)
The hybrid approach resulted in 32 scaffolds, whereas nonhybrid assembly resulted in three scaffolds
Summary
Halolamina pelagica strain CDK2, a halophilic archaeon (growth range 1.36 to 5.12 M NaCl), was isolated from rhizosphere of wild grasses of hypersaline soil of the Rann of Kutch, Gujarat, India. Genomic DNA was extracted and purified using a DNA isolation kit from Zymo Research Laboratories and was sequenced using both the Miseq platform (Illumina) and PacBio SMRT version C5 chemistry. Shotgun sequencing generated 11,512,861 pairedend reads (2 ϫ 250 bp), while PacBio SMRT resulted in 120,716 long reads (30 to 40 kb). The genome was assembled by the Celera Assembler program [14] using two approaches, hybrid (Illumina and PacBio reads) and nonhybrid assembly (PacBio reads).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
