Abstract
The thermophilic Geobacillus sp. strain LEMMJ02 was isolated from Fumarole Bay sediment on Deception Island, an active Antarctic volcano. Here, we report the draft genome of LEMMJ02, which consists of 3,160,938 bp with 52.8% GC content and 3,523 protein-coding genes.
Highlights
The genus Geobacillus is a member of the phylum Firmicutes, consisting of Grampositive, aerobic or facultative anaerobic, heterotrophic, and obligatory thermophilic bacteria [1]
After incubation at 55°C for 48 h, a single colony was used for genomic DNA extraction with a Wizard genomic DNA purification kit (Promega, Madison, USA), and the DNA was quantified using a Qubit fluorometer (Thermo Fisher Scientific, Waltham, USA)
The assembled scaffolds were quality checked with CheckM and RefineM [9], and the coding DNA sequences (CDSs) were predicted and annotated using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) tool [10] and the Rapid Annotations using Subsystems Technology (RAST) server [11]
Summary
The genus Geobacillus is a member of the phylum Firmicutes, consisting of Grampositive, aerobic or facultative anaerobic, heterotrophic, and obligatory thermophilic bacteria [1]. After incubation at 55°C for 48 h, a single colony was used for genomic DNA extraction with a Wizard genomic DNA purification kit (Promega, Madison, USA), and the DNA was quantified using a Qubit fluorometer (Thermo Fisher Scientific, Waltham, USA). A NEBNext Ultra II FS DNA library kit (New England Biolabs, Ipswich, USA) was used to prepare a paired-end 300-bp library, which was sequenced on an Illumina MiSeq system.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
