Abstract
We report the draft genome sequence of Streptomyces sp. strain BPSDS2, isolated from freshwater sediments in Northeast India. The draft genome has a size of 8.27 Mb and 7,559 protein-coding sequences.
Highlights
Streptomyces spp. are Gram-positive filamentous spore-forming bacteria, belong to the most dominant genus of the Actinobacteria, have high GϩC content in their DNA (1), and are remarkably rich sources of bioactive compounds, accounting for the production of over two-thirds of the commercially available antibiotics in current use (2)
Library size distribution was checked on an Agilent TapeStation D1000 DNA chip
The Streptomyces sp. strain BPSDS2 genome was annotated on the PATRIC Web server (9) using the RAST tool kit (RASTtk) (10), and it contains 7,546 protein-coding sequences (CDS), 65 tRNA genes, and 3 rRNA genes. The annotation of this strain consists of 2,498 hypothetical proteins and 5,048 proteins with functional assignments, including 1,233 proteins with Enzyme Commission (EC) numbers, 1,070 with Gene Ontology (GO) assignments, and 968 proteins that were mapped to the KEGG pathway
Summary
Streptomyces spp. are Gram-positive filamentous spore-forming bacteria, belong to the most dominant genus of the Actinobacteria, have high GϩC content in their DNA (1), and are remarkably rich sources of bioactive compounds, accounting for the production of over two-thirds of the commercially available antibiotics in current use (2). 500 ng of the isolated good-quality genomic DNA was fragmented using a Covaris M220 sonicator. The fragmented genomic DNA was end repaired and further processed for ligation of Illumina adaptors with the NEBNext Ultra DNA library preparation kit (catalog number E7370L; NEB), as per the recommendation of the manufacturer.
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