Abstract
We announce the availability of the 5.023-Mbp high-quality draft assembly of the Escherichia coli strain Nissle 1917 (serovar O6:K5:H1) genome. Short genomic segments from this important probiotic strain have been available in public databases, but the full genome sequence has remained inaccessible. Thus, high-coverage, whole genome sequencing of E. coli Nissle 1917 is presented herein. Reannotation and metabolic reconstruction will enable comparative genomics analysis and model-guided predictions of genetic manipulations leading to increased production of the K5 capsular polysaccharide known as N-acetyl heparosan, a precursor to the anticoagulant pharmaceutical heparin.
Highlights
We announce the availability of the 5.023-Mbp high-quality draft assembly of the Escherichia coli strain Nissle 1917 genome
Serotyping of E. coli Nissle 1917 has identified the presence of a K5 antigen, which is known to be composed of N-acetyl heparosan, a group 2 capsular polysaccharide (CPS) consisting of a repeating [¡4) -D-glucuronic acid (GlcA) (1 ¡ 4) N-acetyl-␣-D-glucosamine (GlcNAc) (1¡]n disaccharide unit (5)
Escherichia coli Nissle 1917 was cultured from a Mutaflor tablet (Ardeypharm, Herdecke, Germany) and plated on Luria-Bertani (LB) agar medium; a single colony was picked and grown in LB medium at 37°C overnight, and the genomic DNA was purified with an Invitrogen PureLink genomic DNA mini kit
Summary
We announce the availability of the 5.023-Mbp high-quality draft assembly of the Escherichia coli strain Nissle 1917 (serovar O6:K5:H1) genome. The commensal Escherichia coli strain Nissle 1917 is one of the oldest, most well-characterized probiotic agents and has shown promising results in treatment of various intestinal diseases and disorders (1) since its isolation from the feces of a World War I soldier exhibiting unique intestinal fortitude compared to his cohort, all of whom developed infectious diarrhea (2). Assorted genomics studies have been performed on Nissle 1917, including tRNA screening, genomic island sequencing, DNA-DNA hybridization (3), and even low-coverage genomic shotgun sequencing (4).
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