Abstract
Cronobacter sakazakii is an emerging pathogen that causes meningitis, bacteremia, sepsis, and necrotizing enterocolitis in premature infants. Strain Cr268 was isolated from imported powdered infant formula in 2009 during routine microbial examination according to ISO-22964 ("Microbiology of the food chain-horizontal method for the detection of Cronobacter spp."). Isolate Cr268 was confirmed to be C. sakazakii by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and standard biochemical analysis. Here, we announce its genome, which represents a new member in the C. sakazakii group.
Highlights
Cronobacter sakazakii is an emerging pathogen that causes meningitis, bacteremia, sepsis, and necrotizing enterocolitis in premature infants
The isolate was revived from a frozen stock on Trypticase soy agar (TSA) at 37°C for 24 h
Remapping the reads to the de novo assembly resulted in a median base coverage of 68ϫ and a mean base coverage of 68.8ϫ
Summary
Cronobacter sakazakii is an emerging pathogen that causes meningitis, bacteremia, sepsis, and necrotizing enterocolitis in premature infants. The isolate was revived from a frozen stock on Trypticase soy agar (TSA) at 37°C for 24 h. Genomic DNA of C. sakazakii Cr268 was purified using a MagNA Pure Compact instrument (Roche) following the manufacturer’s instructions. DNA libraries were prepared using a Nextera DNA Flex library preparation kit following the manufacturer’s instructions. Sequencing was performed on an Illumina MiSeq platform using a 250-bp paired-end read v2 kit.
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