Draft Genome Sequence of Commercial Textile Dye-Decolorizing and -Degrading Bacillus subtilis Strain C3 Isolated in India.

Khushbu Kunadia,Prakash G Koringa,Priyank A Patel,Ramesh K Kothari,Chaitanya G Joshi,Charmy R Kothari,Jalpa K Rank,Neelam M Nathani,Anjali Joshi,Vishal Kothari,Divyarajsinh Jadeja,M Chandra Shekar,Rohit J Kotadia,Ravindra Pal Singh,Priti R Faldu,Bhaskar Reddy,Mitkumar J Viroja

doi:10.1128/genomea.00104-16

Abstract

Bacillus subtilis C3, a commercial textile dye-decolorizing and -degrading bacterium, was isolated from the common effluent treatment plant (CEPT) of the Jetpur textile dyeing and printing industrial sector situated in the district of Rajkot, Gujarat, India. Here, we present the annotated 4.18-Mb draft genome sequence of B. subtilis C3, providing information about the metabolic pathways involved in decolorization and degradation of several commercial textile azo dyes. Thus, we confirm B. subtilis C3 as a potential candidate for bioremediation of textile effluents.

Highlights

Bacillus subtilis C3, a commercial textile dye-decolorizing and -degrading bacterium, was isolated from the common effluent treatment plant (CEPT) of the Jetpur textile dyeing and printing industrial sector situated in the district of Rajkot, Gujarat, India
Bacillus subtilis C3 isolated from a common effluent treatment plant (CEPT) exhibited ~95% to 100% decolorization of various azo dyes during 24 to 48 h of incubation under static culture conditions
The draft genome revealed the presence of 4,972 coding sequences (CDS), including genes encoding enzymes involved in azo reduction, supporting the potential of the strain as a potent candidate for decolorization and biodegradation of textile dyes and degradation of benzoate and catechol

Summary

Introduction

Bacillus subtilis C3, a commercial textile dye-decolorizing and -degrading bacterium, was isolated from the common effluent treatment plant (CEPT) of the Jetpur textile dyeing and printing industrial sector situated in the district of Rajkot, Gujarat, India. Several industries make use of dyes and pigments to color products such as textiles, tannery materials, food, paper and pulp, printing materials, carpet, etc. 15% to 20% of these synthetic dyes enter the environment through effluents generated during the manufacture and processing operations, and the disposal of these wastes into receiving waters causes damage to the environment (2).

Results

Conclusion