Draft Genome Sequence of Cellulosilyticum sp. I15G10I2, a Novel Bacterium Isolated from a Coal Seam Gas Water Treatment Pond.

Joseph Adelskov,Bharat K C Patel

doi:10.1128/genomea.01616-16

Abstract

ABSTRACTCellulosilyticum sp. strain I15G10I2 was isolated from a coal seam gas water treatment pond at the Spring Gully water treatment facility, Roma, Queensland, Australia. Analysis of the genome of 4,489,861 bp and G+C content of 35.23% revealed that strain I15G10I2 shared limited similarity to members of the genus Cellulosilyticum, family Lachnospiraceae.

Highlights

Cellulosilyticum sp. strain I15G10I2 was isolated from a coal seam gas water treatment pond at the Spring Gully water treatment facility, Roma, Queensland, Australia
High-molecular-weight DNA of strain I15G10I2 was extracted using a modification of Marmur’s method [5] and submitted to the Australian Genomic Research Facility (AGRF) for TruSeq library preparation and sequencing on the Illumina MiSeq platform with specifications set for paired-end (PE) sequencing (2 ϫ 250-bp read lengths)
Phyla-AMPHORA [10] analysis of 56 universal genes extracted from 186 representative genomes of the family Lachnospiraceae confirmed that strain I15G10I2 was most closely related to members of the genus Cellulosilyticum, but ANIb and the Genome-to-Genome Distance Calculator [11] showed a weak relationship, suggesting that it was a new species of the genus

Summary

Introduction

Cellulosilyticum sp. strain I15G10I2 was isolated from a coal seam gas water treatment pond at the Spring Gully water treatment facility, Roma, Queensland, Australia. High-molecular-weight DNA of strain I15G10I2 was extracted using a modification of Marmur’s method [5] and submitted to the Australian Genomic Research Facility (AGRF) for TruSeq library preparation and sequencing on the Illumina MiSeq platform with specifications set for paired-end (PE) sequencing (2 ϫ 250-bp read lengths).

Results

Conclusion