Draft Genome Sequence of Brevundimonas sp. Strain T2.26MG-97, Isolated from a Rock Core Sample from 492.6 Meters Deep on the Subsurface of the Iberian Pyrite Belt.

E Rodríguez-Robles,T Leandro,R Amils,J M Martínez

doi:10.1128/mra.00375-19

Abstract

The draft genome of Brevundimonas sp. strain T2.26MG-97, isolated at a depth of 492.6 m in the subsurface of the Iberian Pyrite Belt, is reported here. It consists of 262 scaffolds with a total genome length of 3.68 Mbp, where 3,549 coding DNA sequences have been annotated.

Highlights

The genus Brevundimonas is classified within the class Alphaproteobacteria and family Caulobacteraceae
For whole-genome sequencing, genomic DNA was extracted by the cetyltrimethylammonium bromide (CTAB)-based method (10), and its concentration was determined with a Qubit v.2.0 fluorometer (Invitrogen, USA)
It was submitted to the MicrobesNG sequencing facility (University of Birmingham, UK) for Illumina MiSeq sequencing with 27ϫ coverage

Summary

Introduction

The genus Brevundimonas is classified within the class Alphaproteobacteria and family Caulobacteraceae. For whole-genome sequencing, genomic DNA was extracted by the cetyltrimethylammonium bromide (CTAB)-based method (10), and its concentration was determined with a Qubit v.2.0 fluorometer (Invitrogen, USA). It was submitted to the MicrobesNG sequencing facility (University of Birmingham, UK) for Illumina MiSeq sequencing with 27ϫ coverage. A Nextera XT kit (Illumina) was used to prepare the genomic libraries following the manufacturer’s protocol.

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Conclusion