Abstract
Bacillus safensis strain JG-B5T was isolated from soil of the uranium mining waste pile Haberland located near Johanngeorgenstadt, Saxony, Germany. We report here a draft genome sequence (3.7 Mb) of this bacterial strain. The high metal resistance abilities of B. safensis strain JG-B5T can be exploited for bioremediation of metal and metalloid-contaminated environments.
Highlights
Bacillus safensis strain JG-B5T was isolated from soil of the uranium mining waste pile Haberland located near Johanngeorgenstadt, Saxony, Germany
The pure culture of B. safensis strain JG-B5T was routinely cultivated in nutrient broth (10 g/liter; Mast Group Ltd., Merseyside, UK) at 30°C
AROS Applied Biotechnology A/S (Eurofins Genomics, Denmark) performed wholegenome sequencing of 200 ng of double-stranded DNA using next-generation sequencing technology with the Illumina HiSeq 2000 platform; 100-bp paired-end reads were used for whole-genome sequencing with a run time of 8 days
Summary
Bacillus safensis strain JG-B5T was isolated from soil of the uranium mining waste pile Haberland located near Johanngeorgenstadt, Saxony, Germany. The pure culture of B. safensis strain JG-B5T was routinely cultivated in nutrient broth (10 g/liter; Mast Group Ltd., Merseyside, UK) at 30°C. Genomic DNA of B. safensis strain JG-B5T was extracted using the MasterPure Gram-positive DNA purification kit (Epicentre) according to the manufacturer’s instructions. Purity and concentration of the DNA were determined using the NanoDrop 2000/2000c UV/Vis spectrophotometer (Thermo Scientific) and agarose gel electrophoresis (7).
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