Abstract
Here, we report the draft genome sequence of Bacillus cereus strain UAEU-H3K6M1, which was isolated from petroleum sludge in the desert. It is composed of around 5.4 Mbp and has a GC content of 35%. Functional annotation and pathway modeling showed multiple genes with potential bioremediation abilities.
Highlights
We report the draft genome sequence of Bacillus cereus strain UAEU-H3K6M1, which was isolated from petroleum sludge in the desert
Strain UAEU-H3K6M1 was selected for genome analysis based on the criteria described above
The most common subsystems identified by rapid genome annotations using RAST (9) were those related to amino acids and derivatives (n ϭ 495 coding sequences [CDSs]), carbohydrates (n ϭ 421), cofactors, vitamins, prosthetic groups, and pigments (n ϭ 223), cell wall and capsule (n ϭ 169), RNA metabolism (n ϭ 149), membrane transport (n ϭ 147), fatty acids, lipids, and isoprenoids (n ϭ 140), protein metabolism (n ϭ 109), nucleosides and nucleotides (n ϭ 107), and defense (n ϭ 105)
Summary
We report the draft genome sequence of Bacillus cereus strain UAEU-H3K6M1, which was isolated from petroleum sludge in the desert. Strain UAEU-H3K6M1 was selected for genome analysis based on the criteria described above. An overnight culture (37°C with gentle shaking) of this strain in nutrient broth was used to isolate genomic DNA using an ABIOPure DNA isolation kit (Alliance Bio, USA).
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