Draft Genome Sequence of Aquincola tertiaricarbonis MIMtkpLc11, an Aerobic Anoxygenic Phototrophic Bacterial Strain Isolated from Biological Soil Crusts.

Kai Tang,Lijuan Jia,Fuying Feng,Yonghui Zeng,Bo Yuan

doi:10.1128/mra.01085-18

Abstract

Aquincola tertiaricarbonis strain MIMtkpLc11 was isolated from biological soil crusts in Inner Mongolia, China. The strain contains photosynthesis gene clusters. Here, we report the draft genome sequence of strain MIMtkpLc11, which comprises 98 contigs (N 50, 233,472 bp) and 5,573 protein-coding sequences.

Highlights

Aquincola tertiaricarbonis strain MIMtkpLc11 was isolated from biological soil crusts in Inner Mongolia, China
The open reading frames (ORFs) of the assembled genome were predicted with Glimmer version 3.02 (13)
All predicted ORFs were searched with BLAST version 2.2.30ϩ (14) against all proteins from complete microbial genomes in the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) version 3.1 (15)

Summary

Introduction

Aquincola tertiaricarbonis strain MIMtkpLc11 was isolated from biological soil crusts in Inner Mongolia, China. Genomic DNA was extracted using a genomic DNA purification kit (Tiangen Biotech Co., Beijing, China) and fragmented to sizes of 300 to 500 bp using the M220 ultrasonicator (Covaris, USA). Paired-end libraries (300-bp length) were prepared using a TruSeq DNA sample prep kit (Illumina, Inc., USA) and sequenced using the Illumina HiSeq 2000 system.

Results

Conclusion