Abstract
ObjectiveA Paenibacillus strain isolated in previous research exhibited antimicrobial activity against relevant human pathogens including Staphylococcus aureus and Listeria monocytogenes. In this study, the genome of the aforementioned strain, designated as MP1, was shotgun sequenced. The draft genome of strain MP1 was subject to multiple genomic analyses to taxonomically characterize it and identify the genes potentially responsible for its antimicrobial activity.ResultsHere we report the draft genome sequence of an antimicrobial producing Paenibacillus strain, MP1. Average Nucleotide Identity (ANI) analysis established strain MP1 as a new strain of the previously characterized Paenibacillus alvei. The genomic analysis identified several putative secondary metabolite clusters including seven Nonribosomal Peptide Synthetase clusters (NRPS) (> 10,000 nt), one bacteriocin or other unspecified Ribosomally Synthesized and Post-Translationally modified Peptide Product (RiPP), one lanthipeptide, and six hybrid clusters (NRPS-Type I Polyketide synthase (T1PKS) and NRPS-trans Amino Transferase Polyketide Synthase (AT-PKS)).
Highlights
Paenibacillus spp. produce a variety of peptide and nonpeptide-containing antimicrobial compounds
When compared to other strains within the species P. alvei, MP1 was more distantly related: B-LR (84.89%, GCF_900519125.1), TS-15 (84.78%, GCF_000442555.1), and A6-6i-x (84.60%, GCF_000442535.1) (Additional file 3). These values are higher than the Average Nucleotide Identity (ANI) values between strain MP1 and other Paenibacillus type strains
The ANI value between MP1 and type strain P. alvei DSM 29 indicates that strain MP1 is a new strain within the species Paenibacillus alvei when considering the 95% threshold suggested by others [28, 29]
Summary
The resulting assembly of strain MP1 contained 116 scaffolds with an N 50 value of 129,056 bp, 46.11% G + C content, and 6,511,289 bp; average sequencing coverage was 106.03 × (Additional file 1: Summary Quast Table). ANI analysis showed strain MP1 was most closely related to type strain P. alvei DSM 29 (97.68%, refseq accession GCF_000293805.1) (Additional file 2). When compared to other strains within the species P. alvei, MP1 was more distantly related: B-LR (84.89%, GCF_900519125.1), TS-15 (84.78%, GCF_000442555.1), and A6-6i-x (84.60%, GCF_000442535.1) (Additional file 3) These values are higher than the ANI values between strain MP1 and other Paenibacillus type strains When the translated sequences of these genes were compared to related proteins using BLASTp, they displayed high similarity to proteins involved in the synthesis of variant forms of polymyxin B (1 and 2) produced by a strain of P. polymyxa [25]: PmxA (89.15%, AEZ51516.1), PmxB (96.28%, AEZ51517.1), PmxC (95.89%, AEZ51518.1), PmxD When the translated sequences of these genes were compared to related proteins using BLASTp, they displayed similarity to proteins involved in the synthesis of paenibactin produced by a strain of P. elgii [27]: PaeG (59.64%, AEI70240.1), PaeA (75.00%, AEI70241.1), PaeC (58.17%, AEI70242.1), PaeE (77.09%, AEI70243.1), PaeB (66.56%, AEI70244.1), and PaeF (67.78%, AEI70245.1)
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