Abstract
Fusarium proliferatum is a component of the onion basal rot disease complex. We present an annotated F. proliferatum draft genome sequence, totaling 45.8 Mb in size, assembled into 597 contigs, with a predicted 15,418 genes. The genome contains 58 secondary metabolite clusters and homologs of the Fusarium oxysporum effector SIX2.
Highlights
Fusarium proliferatum is a component of the onion basal rot disease complex
Published RNA sequencing (RNA-seq) data [7] were aligned to the genome using Bowtie 2 version 2.2.4 and TopHat version 2.1.0 [12, 13], with mate-inner-dist set to Ϫ20 bp and mate-std-dev set to 70 bp
These alignments were used in the prediction of 15,418 genes encoding 15,448 proteins; 15,421 of these proteins were predicted using BRAKER1 version 2.0 [14], supplemented by an additional 37 proteins predicted by using CodingQuarry version 2.0 [15], located in intergenic regions of BRAKER1 gene models
Summary
Fusarium proliferatum is a component of the onion basal rot disease complex. We present an annotated F. proliferatum draft genome sequence, totaling 45.8 Mb in size, assembled into 597 contigs, with a predicted 15,418 genes. Reads were trimmed and adapters removed using fastq-mcf version 1.04.676 [9] before a 45.8-Mb genome assembly was generated using SPAdes version 3.5.0 [10] in 581 contigs (Table 1). This masked 1.21 Mb of the genome.
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