Draft Genome Sequence of an Enterococcus faecalis ATCC 19433 Siphovirus Isolated from Raw Domestic Sewage.

Tasha M Santiago-Rodriguez,Gary A Toranzos,Melissa Ly,David T Pride

doi:10.1128/genomea.01179-16

Abstract

ABSTRACTWe previously isolated and characterized an Enterococcus faecalis ATCC 19433 siphovirus from raw domestic sewage as a viral indicator of human fecal pollution. Here, we report the draft genome sequence of this bacteriophage.

Highlights

We previously isolated and characterized an Enterococcus faecalis ATCC 19433 siphovirus from raw domestic sewage as a viral indicator of human fecal pollution
One milliliter of the cesium chloride (CsCl) fraction was purified on Amicon YM-100 protein columns (Millipore, Billerica, MA) and treated with DNase I
Reads were trimmed according to modified Phred scores of 0.5 using CLC Genomics Workbench 4.65 (Cambridge, MA, USA)

Summary

Introduction

We previously isolated and characterized an Enterococcus faecalis ATCC 19433 siphovirus from raw domestic sewage as a viral indicator of human fecal pollution. One milliliter (109 PFU/ml) of the E. faecalis ATCC 19433 phage was filtered sequentially using 0.45- and 0.2-␮m filters (VWR, Radnor, PA, USA) and purified on a cesium chloride (CsCl) density gradient [3]. One milliliter of the CsCl fraction was purified on Amicon YM-100 protein columns (Millipore, Billerica, MA) and treated with DNase I. DNA was isolated using a Qiagen UltraSens virus kit (Qiagen, Valencia, CA, USA), amplified using GenomiPhi HY MDA (GE Healthcare, Pittsburgh, PA, USA), and fragmented to 200 to 400 bp using a Bioruptor (Diagenode, Denville, NJ, USA).

Results

Conclusion