Draft Genome Sequence of Agrobacterium rhizogenes Strain NCPPB2659.

Jose A Valdes Franco,James G Thomson,Yong Gu,Ray Collier,Roger Thilmony,Naxin Huo,Yi Wang

doi:10.1128/genomea.00746-16

Jose A Valdes Franco, James G Thomson + Show 5 more

Open Access

https://doi.org/10.1128/genomea.00746-16

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Abstract

This work reports the draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659 (also known as strain K599). The assembled genome contains 5,277,347 bp, composed of one circular chromosome, the pRi2659 virulence plasmid, and 17 scaffolds pertaining to the linear chromosome. The wild-type strain causes hairy root disease in dicots and has been used to make transgenic hairy root cultures and composite plants (nontransgenic shoots with transgenic roots). Disarmed variants of the strain have been used to produce stable transgenic monocot and dicot plants.

Highlights

This work reports the draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659
A. rhizogenes strain NCPPB2659 induces hairy roots from transformationrecalcitrant plants, like soybean, with higher efficiency relative to other strains [6], and this characteristic has been used for gene function studies in hairy root cultures [7, 8] and composite plants [9]
To enhance the stability of constructs containing large synthetic transfer DNA (T-DNA) molecules in the wild-type and disarmed strains, the genome sequence data were used to design a cassette for the TcR insertional inactivation of recA [13, 14]

Summary

Introduction

This work reports the draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659 ( known as strain K599). Agrobacterium rhizogenes is a Gram-negative soil bacterial plant pathogen that causes hairy root disease [1] resulting from in planta expression of rol genes [2] after transfer DNA (T-DNA) movement into the host plant cells [3]. The Ri T-DNA of A. rhizogenes strain NCPPB2659, isolated from cucumbers displaying hairy root disease, was characterized [4], and the pRi2659 virulence plasmid sequenced [5]. To enhance the stability of constructs containing large synthetic T-DNA molecules in the wild-type and disarmed strains (such as A. rhizogenes 18r12v), the genome sequence data were used to design a cassette for the TcR insertional inactivation of recA [13, 14].

Results

Conclusion