Draft Genome Sequence of Acinetobacter calcoaceticus Strain P23, a Plant Growth-Promoting Bacterium of Duckweed.

Masayuki Sugawara,Masaaki Morikawa,Akira Hosoyama,Atsushi Yamazoe

doi:10.1128/genomea.00026-15

Abstract

Acinetobacter calcoaceticus strain P23 is a plant growth-promoting bacterium, which was isolated from the surface of duckweed. We report here the draft genome sequence of strain P23. The genome data will serve as a valuable reference for understanding the molecular mechanism of plant growth promotion in aquatic plants.

Highlights

Acinetobacter calcoaceticus strain P23 was isolated as a phenoldegrading bacterium from the surface of a species of duckweed (Lemna aoukikusa) grown in the Hokkaido University Botanical Garden, Japan [1]
The genome of strain P23 was sequenced by single-end sequencing with 454 GS FLXϩ (Roche, Basel, Switzerland) and paired-end sequencing with the Illumina HiSeq 1000 sequencing system (Illumina, San Diego, CA, USA)
The P23 draft genome, which is 4,058,823 bp in length (38.5% GϩC content), includes 3,730 protein-coding sequences (CDS), 3 rRNA genes, and 61 tRNA genes distributed in a total of 44 contigs (Ͼ100 bp)

Summary

Introduction

Acinetobacter calcoaceticus strain P23 was isolated as a phenoldegrading bacterium from the surface of a species of duckweed (Lemna aoukikusa) grown in the Hokkaido University Botanical Garden, Japan [1]. It was previously observed that the bacterium colonizes on root surfaces and promotes the growth of duckweeds, including L. aoukikusa and Lemna minor [1, 2]. The strain increases the chlorophyll content in monocot duckweed and dicot lettuce (Lactuca sativa) in a hydroponic culture [2].

Results

Conclusion