Abstract
ABSTRACTThe use of halotolerant acidophiles for bioleaching provides a biotechnical approach for the extraction of metals from regions where high salinity exists in the ores and source water. Here, we describe the first draft genome of a new species of a halotolerant and iron- and sulfur-oxidizing acidophile, Acidihalobacter ferrooxidans DSM-14175 (strain V8).
Highlights
The use of halotolerant acidophiles for bioleaching provides a biotechnical approach for the extraction of metals from regions where high salinity exists in the ores and source water
Total DNA was extracted from A. ferrooxidans DSM 14175 using the modified method of nucleic acid extraction for acidophiles, as described by Zammit et al [5]
Similar to the genomes of A. prosperus DSM 5130 and DSM 14174, genome analysis of A. ferrooxidans DSM 14175 showed the presence of the rus operon genes for iron oxidation [8,9,10]
Summary
The use of halotolerant acidophiles for bioleaching provides a biotechnical approach for the extraction of metals from regions where high salinity exists in the ores and source water. Total DNA was extracted from A. ferrooxidans DSM 14175 using the modified method of nucleic acid extraction for acidophiles, as described by Zammit et al [5]. DNA was sequenced using Illumina MiSeq (619,160 paired-end reads, 2 ϫ 300-bp reads) and PacBio RS SMRT sequencing technologies (733,419 subreads with a mean read length of 1,602 bp).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
